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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
11/08/2016 |
Actualizado : |
12/03/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
ROCHA, C.M.L.; VELLICCE, G.R.; GARCÍA, M.G.; PARDO, E.M.; RACEDO, J.; PERERA, M.F.; DE LUCÍA, A.; BONNECARRERE, M.; GERMAN, S.; MARCELINO, F.; LEDESMA, F.; REZNIKOV, S.; PLOPER, D.L.; WELIN, B.; CASTAGNARO, A.P. |
Afiliación : |
CARLA MARÍA LOURDES ROCHA, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; GABRIEL RICARDO VELLICCE, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; MARÍA GABRIELA GARCÍA, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; ESTEBAN MARIANO PARDO, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; JOSEFINA RACEDO, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; MARÍA FRANCISCA PERERA, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; ADRIÁN DE LUCÍA, INTA (Instituto Nacional de Tecnología Agropecuaria) - Cerro Azul; MARIA VICTORIA BONNECARRERE MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVIA ELISA GERMAN FAEDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANCISMAR MARCELINO, EMBRAPA Soja; FRANCISCO LEDESMA, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; SEBASTIÁN REZNIKOV, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; LEONARDO DANIEL PLOPER, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; BJORN WELIN, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; ATILIO PEDRO CASTAGNARO, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino. |
Título : |
Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi. |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
Electronic Journal of Biotechnology, 2015, v. 18, no. 6, p. 439-444. OPEN ACCESS. |
DOI : |
10.1016/j.ejbt.2015.06.007 |
Idioma : |
Inglés |
Notas : |
Article history: Received 24 April 2015. Accepted 23 September 2015. Available online 28 October 2015. |
Contenido : |
ABSTRACT.
Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919
markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies.
© 2015 Pontificia Universidad Católica de Valparaíso. Production and hosting by Elsevier B.V. All rights reserve MenosABSTRACT.
Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919
markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an impor... Presentar Todo |
Palabras claves : |
ASIAN SOYBEAN RUST; GENETIC VARIATION; GLYCINE MAX; MOLECULAR MARKERS. |
Thesagro : |
MARCADORES MOLECULARES; SOJA; VARIACION GENETICA. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/5850/1/Rocha-C.M.-2015.-Electr.Jr.Biotech.-v18-p.439-444.pdf
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Marc : |
LEADER 02957naa a2200397 a 4500 001 1055241 005 2019-03-12 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1016/j.ejbt.2015.06.007$2DOI 100 1 $aROCHA, C.M.L. 245 $aUse of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi.$h[electronic resource] 260 $c2015 500 $aArticle history: Received 24 April 2015. Accepted 23 September 2015. Available online 28 October 2015. 520 $aABSTRACT. Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies. © 2015 Pontificia Universidad Católica de Valparaíso. Production and hosting by Elsevier B.V. All rights reserve 650 $aMARCADORES MOLECULARES 650 $aSOJA 650 $aVARIACION GENETICA 653 $aASIAN SOYBEAN RUST 653 $aGENETIC VARIATION 653 $aGLYCINE MAX 653 $aMOLECULAR MARKERS 700 1 $aVELLICCE, G.R. 700 1 $aGARCÍA, M.G. 700 1 $aPARDO, E.M. 700 1 $aRACEDO, J. 700 1 $aPERERA, M.F. 700 1 $aDE LUCÍA, A. 700 1 $aBONNECARRERE, M. 700 1 $aGERMAN, S. 700 1 $aMARCELINO, F. 700 1 $aLEDESMA, F. 700 1 $aREZNIKOV, S. 700 1 $aPLOPER, D.L. 700 1 $aWELIN, B. 700 1 $aCASTAGNARO, A.P. 773 $tElectronic Journal of Biotechnology, 2015$gv. 18, no. 6, p. 439-444. OPEN ACCESS.
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125. | | BOGLIACCHINI, A.; CHIARAVALLE, W.; ERNST, O.; FERNÁNDEZ, G.; MARTÍNEZ, I.; PEREYRA, S.; PÉREZ BIDEGAIN, M.; PIPPOLO, D.; SAWCHIK, J.; CANTOU, G. Guía de buenas prácticas agrícolas para sistemas con agricultura de secano en Uruguay. Montevideo (Uruguay): MGAP, MESA NACIONAL DE TRIGO, MESA TECNOLÓGICA DE OLEAGINOSOS, MESA NACIONAL DE ENTIDADES DE CEBADA CERVECERA, 2013. 53 p.Biblioteca(s): INIA Treinta y Tres. |
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127. | | GUTIÉRREZ, L.; BERBERIAN, N.; CAPETTINI, F.; FALCIONI, E.; FROS, D.; GERMAN, S.; HAYES, P. M.; HUERTA-ESPINO, J.; HERRERA, S.; PEREYRA, S.; PEREZ, C.; SANDOVAL-ISLAS, S.; SINGH, R.; CASTRO, A. Genome-wide association mapping identifies disease resistance QTLs in barley germplasm from Latin America: chapter 18. In: INTERNATIONAL BARLEY GENETICS SYMPOSIUM, 11., 2012, Hangzhou, CN. Advance in barley sciences: proceedings. Dordrecht: Zhejiang University Press/Springer, 2013. p. 209-215Tipo: Capítulo en Libro Técnico-Científico |
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129. | | GUTIÉRREZ, L.; BERBERIAN, N.M.; GERMÁN, S.; PEREYRA, S.; CAPETTINI, F.; FALCONI, E.; HAYES, P.M.; PÉREZ, C.; SANDOVAL-ISLAS, S.; ORJEDA, G.; NEYRA, E.; GONZA, V.A.; CASTRO, A. Multi-environment multi-QTL association mapping in barley In: INTERNATIONAL ANIMAL AND PLANT GENOME CONFERENCE, 21., 2013, San Diego, CA, US. Genome mapping, tagging and characterization: wheat, barley, oat, and related. P0285. [s.l.: INTLPAG], 2013.Tipo: Trabajos en Congresos/Conferencias |
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130. | | UMPIÉRREZ-FALAICHE, M.; GARMENDIA, G.; PEREYRA, S.; RODRÍGUEZ-HARALAMBIDES, A.; WARD, T.J.; VERO, S. Regional differences in species composition and toxigenic potential among Fusarium head blight isolates from Uruguay indicate a risk of nivalenol contamination in new wheat production areas. International Journal of Food Microbiology, v. 166, n. 1, p. 135-140, 2013 Article history:Received 15 April 2013/Received in revised form 18 June 2013/Accepted 23 June 2013/Available online 1 July 2013.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : A - 1 |
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